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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
16/11/2022 |
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Data da última atualização: |
16/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. K. C. da; SANTOS, M. R. A. dos. |
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Afiliação: |
CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
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Título: |
Induction of somatic embryos in Robusta and Conilon varieties of Coffea canephora. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
International Journal of Development Research, v. 12, n. 10, p. 59734-59737, Oct. 2022. |
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ISSN: |
2230-9926 |
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DOI: |
https://doi.org/10.37118/ijdr.25620.10.2022 |
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Idioma: |
Inglês |
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Conteúdo: |
Clonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. |
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Palavras-Chave: |
Amazônia Ocidental; Cefé Conilon; Clonal propagation; Cotyledonary embryos; Embrapa Rondônia; Embriogênese somática; Porto Velho (RO); Western Amazon. |
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Thesagro: |
Café Robusta; Campo Experimental; Coffea Canephora; Cultura de Tecido. |
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Thesaurus Nal: |
Demonstration farms; Somatic embryogenesis; Tissue culture. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148353/1/cpafro-18835.pdf
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Marc: |
LEADER 02361naa a2200337 a 4500
001 2148353
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
022 $a2230-9926
024 7 $ahttps://doi.org/10.37118/ijdr.25620.10.2022$2DOI
100 1 $aSILVA, C. K. C. da
245 $aInduction of somatic embryos in Robusta and Conilon varieties of Coffea canephora.$h[electronic resource]
260 $c2022
520 $aClonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety.
650 $aDemonstration farms
650 $aSomatic embryogenesis
650 $aTissue culture
650 $aCafé Robusta
650 $aCampo Experimental
650 $aCoffea Canephora
650 $aCultura de Tecido
653 $aAmazônia Ocidental
653 $aCefé Conilon
653 $aClonal propagation
653 $aCotyledonary embryos
653 $aEmbrapa Rondônia
653 $aEmbriogênese somática
653 $aPorto Velho (RO)
653 $aWestern Amazon
700 1 $aSANTOS, M. R. A. dos
773 $tInternational Journal of Development Research$gv. 12, n. 10, p. 59734-59737, Oct. 2022.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
